Fibronectin anticorps

N° du produit ABIN113992

Cet anticorps Lapin Polyclonal détecte spécifiquement Fibronectin dans IF, IHC (p), EIA, IHC (fro) et RIA. Il présente une réactivité envers Humain.

Aperçu rapide pour Fibronectin anticorps (ABIN113992)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Détail du produit anti-Fibronectin anticorps

Specificité: This antibody is specific for Fibronectin (100 %). Collagen type I, III, IV and VI < 0.1 % (RIA at 1/10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Purification: Affinity Chromatography.

Immunogène: Purified fibronectin from human plasma

Information d'application

Indications d'application: Immunofluorescence: 1/80. Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8 °C overnight. ELISA (> / = 1/200). RIA. Positive Control: Human skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered solution without BSA or preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

ID gène: 2335

NCBI Accession: NP_002017

UniProt: P02751